Ethyl pyruvate downregulates tumor necrosis factor alpha and interleukin (IL)-6 and upregulates IL-10 in lipopolysaccharide-stimulated canine peripheral blood mononuclear cells

J Vet Med Sci. 2010 Oct;72(10):1379-81. doi: 10.1292/jvms.09-0590. Epub 2010 May 21.

Abstract

We investigated the anti-inflammatory effects of ethyl pyruvate (EP) on LPS-stimulated canine PBMCs in vitro. We found that EP treatment inhibited the mRNA expressions of proinflammatory cytokines (TNF-α and IL-6), but induced mRNA expression of anti-inflammatory cytokines (IL-10). ELISA measurements revealed that EP also effectively downregulates the LPS-induced increase in proinflammatory cytokine release, while upregulating anti-inflammatory cytokine release. These data indicate that EP could be an effective anti-inflammatory agent in dogs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dogs
  • Down-Regulation
  • Flavoring Agents / pharmacology
  • Interleukin-10 / genetics*
  • Interleukin-6 / genetics*
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / physiology*
  • Lipopolysaccharides / pharmacology*
  • Pyruvates / pharmacology*
  • Tumor Necrosis Factor-alpha / drug effects
  • Tumor Necrosis Factor-alpha / genetics*
  • Up-Regulation

Substances

  • Flavoring Agents
  • Interleukin-6
  • Lipopolysaccharides
  • Pyruvates
  • Tumor Necrosis Factor-alpha
  • ethyl pyruvate
  • Interleukin-10