CD34 mediates intestinal inflammation in Salmonella-infected mice

Cell Microbiol. 2010 Nov;12(11):1562-75. doi: 10.1111/j.1462-5822.2010.01488.x.


CD34 is a highly glycosylated sialomucin expressed on a variety of cells, ranging from vascular endothelial cells to haematopoietic stem cells. Depending on its glycosylation state, CD34 has been shown to promote or inhibit cell adhesion and migration; however, a functional role for CD34 in the gut has not been determined. Using a model of Salmonella-induced gastroenteritis, we investigated the role of CD34 in the context of infection. Upon oral infection, the number of CD34+ cells detected in the submucosa, vascular endothelium and lamina propria significantly increased in S. Typhimurium-infected C57Bl/6 mice. The pathology of S. Typhimurium-infected C57Bl/6 mice was characterized by recruitment of neutrophils to the site of inflammation, submucosal oedema and crypt destruction. In contrast, Cd34(-/-) mice showed a delayed pathology, a defect in inflammatory cell migration into the intestinal tissue and enhanced survival. Importantly, this was not due to a lack of chemotactic signals in Cd34(-/-) mice as these mice had either similar or significantly higher levels of pro-inflammatory cytokines and chemokines post infection when compared with infected C57/Bl6 control mice. In summary, we demonstrate a novel role for CD34 in enhancing migration of inflammatory cells and thereby exacerbating host-mediated immunopathology in the intestine of S. Typhimurium-infected mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD34 / immunology*
  • Antigens, CD34 / metabolism
  • Cecum / immunology*
  • Cecum / pathology*
  • Cell Adhesion
  • Cell Movement
  • Chemokines / immunology
  • Edema
  • Endothelium, Vascular / immunology
  • Gastroenteritis / immunology*
  • Gastroenteritis / microbiology
  • Gastroenteritis / pathology
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / pathology
  • Mice
  • Mice, Inbred C57BL
  • Neutrophil Infiltration
  • Salmonella Infections, Animal / immunology*
  • Salmonella Infections, Animal / microbiology
  • Salmonella Infections, Animal / pathology
  • Salmonella typhimurium*


  • Antigens, CD34
  • Chemokines