Simplified method for concentration of mitochondrial membrane protein complexes

Electrophoresis. 2010 Jun;31(12):1934-6. doi: 10.1002/elps.201000019.


Extracting and concentrating mitochondrial protein complexes from gel strips after blue native PAGE (BN-PAGE) can be daunting tasks using the traditional methods, such as electroelution, passive diffusion and centrifugal concentration. We present a simplified gel electrophoresis method to concentrate mitochondrial protein complexes with excellent recovery rate. Mitochondrial complex I present in a long gel strip from BN-PAGE can be easily concentrated into a 0.8 cm gel strip when a second BN-PAGE is performed with a Y-shaped gel and the addition of 0.01% n-dodecyl beta-D-maltoside and 0.001% SDS in the cathode buffer. Once completed, the concentrated protein complex in the gel strip is ready for SDS-PAGE or proteomic studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electron Transport Complex I / chemistry
  • Electron Transport Complex I / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Glucosides / chemistry
  • Mitochondrial Membranes / chemistry*
  • Mitochondrial Proteins / chemistry*
  • Rats
  • Sodium Dodecyl Sulfate / chemistry


  • Glucosides
  • Mitochondrial Proteins
  • Sodium Dodecyl Sulfate
  • dodecyl maltoside
  • Electron Transport Complex I