Trafficking of UL37 proteins into mitochondrion-associated membranes during permissive human cytomegalovirus infection

J Virol. 2010 Aug;84(15):7898-903. doi: 10.1128/JVI.00885-10. Epub 2010 May 26.


Human cytomegalovirus (HCMV) UL37 proteins traffic sequentially from the endoplasmic reticulum (ER) to the mitochondria. In transiently transfected cells, UL37 proteins traffic into the mitochondrion-associated membranes (MAM), the site of contact between the ER and mitochondria. In HCMV-infected cells, the predominant UL37 exon 1 protein, pUL37x1, trafficked into the ER, the MAM, and the mitochondria. Surprisingly, a component of the MAM calcium signaling junction complex, cytosolic Grp75, was increasingly enriched in heavy MAM from HCMV-infected cells. These studies show the first documented case of a herpesvirus protein, HCMV pUL37x1, trafficking into the MAM during permissive infection and HCMV-induced alteration of the MAM protein composition.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cytomegalovirus Infections / pathology*
  • Cytomegalovirus Infections / virology*
  • Endoplasmic Reticulum / chemistry
  • Fibroblasts / virology
  • HSP70 Heat-Shock Proteins / analysis
  • HeLa Cells
  • Humans
  • Immediate-Early Proteins / analysis*
  • Membrane Proteins / analysis
  • Mitochondrial Membranes / chemistry*
  • Protein Transport


  • HSP70 Heat-Shock Proteins
  • Immediate-Early Proteins
  • Membrane Proteins
  • UL37 protein, Human herpesvirus 5
  • glucose-regulated proteins