Dynamics of memory and naïve CD8+ T lymphocytes in humanized NOD/SCID/IL-2Rgammanull mice infected with CCR5-tropic HIV-1

Vaccine. 2010 May 26;28 Suppl 2:B32-7. doi: 10.1016/j.vaccine.2009.10.154.

Abstract

Creating a novel small animal model of HIV-1 infection that can support long-term systemic HIV-1 infection and produce HIV-1-specific immune response has a great benefit for studying HIV-1 pathogenesis in vivo. In the present study, we have generated a humanized mouse, NOG-hCD34 mouse, by transplanting newborn NOD/SCID/IL-2Rgamma(null) mice with human hematopoietic stem cells through hepatic injection. These mice were infected with a CCR5-tropic HIV-1 and were analyzed for plasma viral load, changes in peripheral blood T lymphocytes, and HIV-1-specific antibody production. High level of viral replication, increase in effector/memory CD8(+) T lymphocytes, class-switching to IgG, and production of HIV-1-specific IgGs were observed. Our findings suggest that NOG-hCD34 mice may have a wide variety of application in HIV-1 research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation
  • CD8-Positive T-Lymphocytes / immunology*
  • Cord Blood Stem Cell Transplantation
  • Disease Models, Animal*
  • HIV Antibodies / immunology
  • HIV Infections / immunology*
  • HIV-1 / physiology
  • Humans
  • Immunoglobulin Class Switching
  • Immunoglobulin G / immunology
  • Immunologic Memory*
  • Interleukin Receptor Common gamma Subunit / genetics
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • RNA, Viral / blood
  • Receptors, CCR5 / immunology
  • Viral Load
  • Viremia
  • Virus Replication

Substances

  • HIV Antibodies
  • Immunoglobulin G
  • Interleukin Receptor Common gamma Subunit
  • RNA, Viral
  • Receptors, CCR5