Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr

Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):951-955. doi: 10.1099/ijs.0.022699-0. Epub 2010 May 28.

Abstract

A novel actinomycete, designated strain RM287(T), was isolated from surface-sterilized roots of Samanea saman (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism formed a distinct phyletic line within the radiation of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain RM287(T) was most closely related to Amycolatopsis mediterranei IMSNU 20056(T) (97.4 %), A. rifamycinica DSM 46095(T) (97.2 %), A. kentuckyensis NRRL B-24129(T) (97.2 %), A. pretoriensis DSM 44654(T) (97 %) and A. australiensis DSM 44671(T) (97 %). The novel organism was found to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the dignostic diamino acid in the cell-wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The major menaquinone was MK-9(H(4)). The major fatty acids were iso-C(16 : 0) iso-C(15 : 0), iso 2-OH-C(16 : 0) and iso-C(17 : 0). The DNA G+C content was 71.7 mol%. Phenotypic data clearly distinguished the novel isolate from its closest relatives. The combined genotypic and phenotypic data indicated that strain RM287(T) represented a novel species of the genus Amycolatopsis. The proposed name for this organism is Amycolatopsis samaneae sp. nov., with the type strain RM287(T) ( = TISTR 1919(T) = BCC 35842(T) = NBRC 106095(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry
  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Arabinose / analysis
  • Base Composition
  • Cell Wall / chemistry
  • Cluster Analysis
  • Cytoplasm / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diaminopimelic Acid / analysis
  • Fabaceae / microbiology*
  • Fatty Acids / analysis
  • Galactose / analysis
  • Molecular Sequence Data
  • Phylogeny
  • Plant Roots / microbiology
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Thailand
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Diaminopimelic Acid
  • Arabinose
  • Galactose

Associated data

  • GENBANK/GQ381310