Objective: To establish a rapid fluorescent inhibition test (RFFIT) for testing rabies virus neutralizing antibody and the titer of rabies virus neutralizing antibody. CVS-11 was used as the standard challenge virus, and three generations prepared for the establishment of the virus library.
Methods: International standard for rabies immunoglobulin was used as the reference serum. RFFIT test was established under consulting the protocol of Institute of Pasteur, and its specificity, stability and reproducibility were validated.
Results: We established the RFFIT which showed both good specificity (100%) and reproducibility (P > 0.5).
Conclusion: The establishment of RFFIT test perfected the rabies laboratory techniques and would enhance the overall ability in detecting rabies in China.