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, 25 (6), 875-81

Enhancement of Gastric Ulcer Healing and Angiogenesis by Cochinchina Momordica Seed Extract in Rats

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Enhancement of Gastric Ulcer Healing and Angiogenesis by Cochinchina Momordica Seed Extract in Rats

Jung Mook Kang et al. J Korean Med Sci.

Abstract

Cochinchina momordica seed is the dried ripe seed of Momordica cochinchinensis, a perennial vine. The antiulcer effect of an extract from cochinchina momordica seeds (SK-MS10) was evaluated in a rat model of acetic acid-induced gastric ulcers. Gastric ulcers were produced by subserosal injection of acetic acid. SK-MS10 (200 mg/kg) or vehicle was administered orally once per day for 14 days after the acetic acid injection. The stomach was removed and the ulcer size measured at day 7 and 14 of the treatment. Expression of vascular endothelial growth factor (VEGF) was assessed by real-time RT-PCR and Western blot analysis. In addition, the microvasculature density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry. The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats. The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant. These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.

Keywords: Acetic Acid; Angiogenesis; Cochinchina Momordica Seed Extract; Stomach Ulcer; Vascular Endothelial Growth Factor.

Figures

Fig. 1
Fig. 1
Effects of SK-MS10 on the healing of gastric ulcers. Macroscopic appearance of ulcers generated at the gastric mucosa in the vehicle treated group at 7 and 14 days (A, B) and the SK-MS10 treated group at 7 and 14 days (C, D). Arrows indicate ulcer. (E) Summarized results on changes of the ulcer area in the vehicle and SK-MS10 treated groups. Results are the mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.
Fig. 2
Fig. 2
Effects of SK-MS10 on angiogenesis in the gastric ulcer. Immunochemical staining of microvessels with the von Willebrand factor in the ulcer bases of rats in the vehicle treated group at 7 and 14 days (A, B) and the SK-MS10 treated group at 7 and 14 days (C, D). Note the von Willebrand factor positive cells (dark brown spots indicated by arrows). (E) The number of microvessels at the gastric ulcer bases of the rats in the vehicle and the SK-MS10 treated groups. Results are mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.
Fig. 3
Fig. 3
Effects of SK-MS10 on the expression of VEGF. (A) The relative mRNA expression of VEGF. (B) Western blot analysis for VEGF. Results are mean±SE in 6 animals per group. *P value <0.05 when compared with the vehicle treated group.

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