Serotonin receptor 3A expression in normal and neoplastic B cells

Pathobiology. 2010;77(3):129-35. doi: 10.1159/000292646. Epub 2010 May 28.


Chromosome 11q21-qter is a very gene-rich region and is often targeted by genomic aberrations in a variety of human cancers, including B cell lymphoid neoplasms. In order to identify genes which could be deregulated in diffuse large B cell lymphomas (DLBCL), we analyzed the expression levels of genes mapped on chromosome 11q21-qter across a series of publicly available DLBCL expression data sets. We identified HTR3A, the gene coding for 5-hydroxytryptamine receptor 3A, as the most frequently differentially expressed. Since HTR3A expression had not been previously characterized in normal and neoplastic B cells, we evaluated its expression by real-time PCR and by immunohistochemistry. The gene is expressed by B cells and it seems to be regulated during the mature B cell differentiation, being overexpressed during the germinal center phase. Lymphomas derived from germinal center B cells maintain the expression, with the level of expression varying among different patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / metabolism*
  • B-Lymphocytes / pathology
  • Biopsy
  • Cell Differentiation
  • Cell Line, Tumor
  • Chromosomes, Human, Pair 11
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Lymphoma, Follicular / genetics
  • Lymphoma, Follicular / metabolism*
  • Lymphoma, Follicular / pathology
  • Lymphoma, Large B-Cell, Diffuse / genetics
  • Lymphoma, Large B-Cell, Diffuse / metabolism*
  • Lymphoma, Large B-Cell, Diffuse / pathology
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / analysis
  • Receptors, Serotonin, 5-HT3 / genetics
  • Receptors, Serotonin, 5-HT3 / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction


  • HTR3A protein, human
  • RNA, Messenger
  • Receptors, Serotonin, 5-HT3