Abstract
Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFalpha) are over-expressed in a variety of tumors. Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here, a chimeric protein of EGF and TGFalpha (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA), a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies, which recognize both EGF and TGFalpha. Anti- E5T-mSEA serum at dilution of 1:10 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cancer Vaccines / biosynthesis*
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Cancer Vaccines / immunology
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Cell Line, Tumor
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Enterotoxins / biosynthesis*
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Enterotoxins / genetics
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Epidermal Growth Factor / biosynthesis*
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Epidermal Growth Factor / genetics
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ErbB Receptors / antagonists & inhibitors*
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ErbB Receptors / immunology
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Humans
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Immunization
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Random Allocation
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Transforming Growth Factor alpha / biosynthesis*
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Transforming Growth Factor alpha / genetics
Substances
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Cancer Vaccines
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Enterotoxins
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Recombinant Fusion Proteins
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Transforming Growth Factor alpha
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enterotoxin A, Staphylococcal
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Epidermal Growth Factor
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ErbB Receptors