[Design and activity analysis of chimeric epidermal growth factor fusion vaccine E5T-mSEA]

Sheng Wu Gong Cheng Xue Bao. 2010 Mar;26(3):357-62.
[Article in Chinese]

Abstract

Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFalpha) are over-expressed in a variety of tumors. Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here, a chimeric protein of EGF and TGFalpha (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA), a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies, which recognize both EGF and TGFalpha. Anti- E5T-mSEA serum at dilution of 1:10 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cancer Vaccines / biosynthesis*
  • Cancer Vaccines / immunology
  • Cell Line, Tumor
  • Enterotoxins / biosynthesis*
  • Enterotoxins / genetics
  • Epidermal Growth Factor / biosynthesis*
  • Epidermal Growth Factor / genetics
  • ErbB Receptors / antagonists & inhibitors*
  • ErbB Receptors / immunology
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Humans
  • Immunization
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Random Allocation
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Transforming Growth Factor alpha / biosynthesis*
  • Transforming Growth Factor alpha / genetics

Substances

  • Cancer Vaccines
  • Enterotoxins
  • Recombinant Fusion Proteins
  • Transforming Growth Factor alpha
  • enterotoxin A, Staphylococcal
  • Epidermal Growth Factor
  • ErbB Receptors