Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles

J Virol. 2010 Aug;84(16):8353-8. doi: 10.1128/JVI.00696-10. Epub 2010 Jun 2.


During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy
  • Dengue Virus / isolation & purification
  • Dengue Virus / physiology*
  • Dengue Virus / ultrastructure
  • Immunoprecipitation
  • Viral Envelope Proteins / metabolism*
  • Virion / isolation & purification
  • Virion / ultrastructure*
  • Virus Assembly*


  • Viral Envelope Proteins
  • prM protein, Flavivirus