Age-related changes in glial fibrillary acidic protein mRNA in the mouse brain

Neurobiol Aging. Mar-Apr 1991;12(2):165-70. doi: 10.1016/0197-4580(91)90056-p.


Several RNA sequences were tested for age-related changes in prevalence levels in the mouse cerebral cortex, hippocampus, and cerebellum. In all three regions, there were increased levels of RNA for glial fibrillary acidic protein, an astrocyte-specific protein, by RNA gel-blot analysis and by a solution hybridization assay. There was no change in glutamine synthetase mRNA level, another glial protein. The only other mRNA sequence which changed was Thy-1 antigen, a neuronal protein, which decreased slightly in the hippocampus. We conclude that with age there is an age-related increase in glial fibrillary acidic protein RNA prevalence potentially reflecting an increase in the size, number, and/or fibrous character of astrocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / metabolism*
  • Animals
  • Blotting, Northern
  • Brain Chemistry / physiology*
  • Cerebellum / metabolism
  • Cerebral Cortex / metabolism
  • Glial Fibrillary Acidic Protein / biosynthesis*
  • Glial Fibrillary Acidic Protein / genetics
  • Hippocampus / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nucleic Acid Hybridization
  • RNA Probes
  • RNA, Messenger / metabolism*


  • Glial Fibrillary Acidic Protein
  • RNA Probes
  • RNA, Messenger