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. 1978 Apr 20;508(3):565-70.
doi: 10.1016/0005-2736(78)90100-1.

Specific Release of Plasma Membrane Enzymes by a Phosphatidylinositol-Specific Phospholipase C

Specific Release of Plasma Membrane Enzymes by a Phosphatidylinositol-Specific Phospholipase C

M G Low et al. Biochim Biophys Acta. .

Abstract

The release of plasma membrane ecto-enzymes by a phosphatidylinositol-specific phospholipase C from Staphylococcus aureus was investigated. There was no effect on L-leucyl-beta-naphthylamidase, alkaline phosphodeisterase I and Ca2+- or MG2+-ATPase, but substantial proportions of the alkaline phosphatase and 5-nucleotidase were released. There was no simultaneous release of phospholipid and the solubilized enzymes were not exluded from Sepharose 6-B. It was therefore concluded that release was not a secondary consequence of membrane vesiculation but occurred as a result of the disruption of specific interactions involving the phosphatidylinositol molecule.

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