Gateway Binary Vectors With the Bialaphos Resistance Gene, Bar, as a Selection Marker for Plant Transformation

Biosci Biotechnol Biochem. 2010;74(6):1315-9. doi: 10.1271/bbb.100184. Epub 2010 Jun 7.

Abstract

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / physiology
  • Drug Resistance / genetics*
  • Genes, Plant / genetics*
  • Genetic Engineering / methods*
  • Genetic Markers / genetics
  • Genetic Vectors / genetics*
  • Organophosphorus Compounds / pharmacology*
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics
  • Transformation, Genetic*

Substances

  • Genetic Markers
  • Organophosphorus Compounds
  • bialaphos