Differential substrate specificity of two inositol transporters of Bacillus subtilis

Tetsuro Morinaga; Takatsugu Matsuse; Hitoshi Ashida; Ken-ichi Yoshida

doi:10.1271/bbb.100125

Differential substrate specificity of two inositol transporters of Bacillus subtilis

Biosci Biotechnol Biochem. 2010;74(6):1312-4. doi: 10.1271/bbb.100125. Epub 2010 Jun 7.

Authors

Tetsuro Morinaga¹, Takatsugu Matsuse, Hitoshi Ashida, Ken-ichi Yoshida

Affiliation

¹ Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.

PMID: 20530884
DOI: 10.1271/bbb.100125

Abstract

Bacillus subtilis IolT is the major myo-inositol transporter for growth, while IolF is a minor one unable to support growth. We found that either IolT or IolF was sufficient for moderate growth using D-chiro-inositol. Conversely to IolT, IolF transported D-chiro-inositol more preferentially than myo-inositol. These results indicate that IolT and IolF are different in substrate specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics
Bacillus subtilis / growth & development
Bacillus subtilis / metabolism*
Bacterial Proteins / metabolism*
Biological Transport
Inositol / chemistry
Inositol / metabolism*
Monosaccharide Transport Proteins / metabolism*
Substrate Specificity

Substances

Bacterial Proteins
Monosaccharide Transport Proteins
Inositol