Antioxidant and amine oxidase inhibitory activities of hydroxyurea

Biosci Biotechnol Biochem. 2010;74(6):1256-60. doi: 10.1271/bbb.100096. Epub 2010 Jun 7.

Abstract

Hydroxyurea (HU, NH(2)CONHOH), or hydroxycarbamide, is a hydroxamic acid derivative used as a drug for anti-neoplasm and sickle-cell disease. In this study, HU was found to have antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals and dose-dependent inhibitory activities against monoamine oxidase (MAO)-A, MAO-B, and semicarbazide-sensitive amine oxidase (SSAO) as compared to controls of clorgyline, deprenyl, and semicarbazide respectively. HU showed mixed-type, competitive-type, and competitive-type inhibition, respectively, with respect to substrates of MAO-A, MAO-B, and SSAO with apparent inhibition constants (Ki) of 19.46, 5.38, and 1.84 microM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biphenyl Compounds / chemistry
  • Cattle
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology*
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology*
  • Hydroxyl Radical / chemistry
  • Hydroxyurea / chemistry
  • Hydroxyurea / pharmacology*
  • Kinetics
  • Oxidoreductases Acting on CH-NH2 Group Donors / antagonists & inhibitors*
  • Oxidoreductases Acting on CH-NH2 Group Donors / metabolism
  • Picrates / chemistry

Substances

  • Biphenyl Compounds
  • Enzyme Inhibitors
  • Free Radical Scavengers
  • Picrates
  • Hydroxyl Radical
  • 1,1-diphenyl-2-picrylhydrazyl
  • Oxidoreductases Acting on CH-NH2 Group Donors
  • Hydroxyurea