Chromatin-associated glycoproteins of normal rat liver and Novikoff hepatoma ascites cells

Cancer Res. 1978 Apr;38(4):1052-6.


Glycoproteins were demonstrated in the 0.6 M NaCl extract of chromatin of normal liver and Novikoff hepatoma cells by periodic acid-Schiff staining of sodium dodecyl sulfate-polyacrylamide gels. In chromatin extracts of Novikoff hepatoma cells, six major periodic acid-Schiff-positive bands coincident with Coomassie Blue-stained protein bands were found with molecular weights of 30,000, 54,000, 64,000, 75,000, 104,000, and 127,000. A corresponding extract from normal rat liver chromatin revealed the presence of four major periodic acid-Schiff-positive bands that migrated with protein bands at molecular weights of 16,000, 30,000, 54,000, and 75,000. The glycoproteins of these extracts contained either mannose or alpha-D-glucose, fucose, and N-acetylglucosamine as shown by the specificity of lectins in affinoelectrophoresis. One of the glycoproteins detected by affinoelectrophoresis was very basic.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular / analysis*
  • Chromatin / analysis*
  • Electrophoresis
  • Glycoproteins / analysis*
  • Lectins
  • Liver / analysis*
  • Liver Neoplasms / analysis*
  • Molecular Weight
  • Neoplasm Proteins / analysis*
  • Neoplasms, Experimental / analysis
  • Periodic Acid-Schiff Reaction
  • Rats


  • Chromatin
  • Glycoproteins
  • Lectins
  • Neoplasm Proteins