Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway

Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11841-6. doi: 10.1073/pnas.1003953107. Epub 2010 Jun 1.


Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell-specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicase domain in mice renders both MILI and MIWI2 devoid of piRNAs. Absence of a functional piRNA pathway in Mov10l1 mutant testes causes loss of DNA methylation and subsequent derepression of retrotransposons in germ cells. The Mov10l1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Argonaute Proteins
  • Base Sequence
  • Cell Cycle Proteins
  • DNA Methylation
  • DNA Primers / genetics
  • Fertility
  • Male
  • Meiosis
  • Mice
  • Mice, Knockout
  • Mutation
  • Proteins / metabolism
  • RNA Helicases / deficiency
  • RNA Helicases / genetics*
  • RNA Helicases / metabolism*
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism*
  • Retroelements / genetics
  • Ribonucleoproteins, Small Nuclear / genetics
  • Ribonucleoproteins, Small Nuclear / metabolism
  • Spermatocytes / metabolism
  • Spermatogenesis
  • Spermatogonia / metabolism
  • Testis / metabolism


  • Argonaute Proteins
  • Cell Cycle Proteins
  • DNA Primers
  • Piwil1 protein, mouse
  • Piwil2 protein, mouse
  • Proteins
  • RNA, Small Interfering
  • Retroelements
  • Ribonucleoproteins, Small Nuclear
  • Tdrd1 protein, mouse
  • Mov10l1 protein, mouse
  • RNA Helicases

Associated data

  • GEO/GSE21763