Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Sep;66(3):197-203.
doi: 10.1007/s13105-010-0024-8. Epub 2010 Jun 10.

Effect of Clenbuterol on Apoptosis, Adipogenesis, and Lipolysis in Adipocytes


Effect of Clenbuterol on Apoptosis, Adipogenesis, and Lipolysis in Adipocytes

Hye-Kyeong Kim et al. J Physiol Biochem. .


Clenbuterol, a beta(2)-adrenergic receptor (beta(2)-AR) selective agonist, has been shown to decrease body fat in animals and can induce apoptosis in adipose tissue in mice. We hypothesized that direct actions of a beta-adrenergic receptor agonist on adipocytes could trigger the observed apoptotic effect. The hypothesis was inspected by investigating the direct effect of clenbuterol on apoptosis, adipogenesis, and lipolysis in vitro using the 3T3-L1 cell line and rat primary adipocytes. Cells were treated with 10(-9) to 10(-5) M clenbuterol depending on the experiments. There was no apoptotic effect of clenbuterol both in 3T3-L1 cells and rat primary adipocytes. Adipogenesis monitored by Oil Red O staining and AdipoRed assay was modestly decreased by clenbuterol treatment (p < 0.05). In fully differentiated primary adipocytes, clenbuterol increased basal lipolysis compared with the control (p < 0.01). In summary, direct stimulation of beta(2)-AR by clenbuterol does not cause apoptosis in adipocytes, despite a direct lipolytic stimulation and attenuation of adipogenesis.

Similar articles

See all similar articles

Cited by 6 articles

See all "Cited by" articles


    1. J Biol Chem. 1997 Oct 10;272(41):25913-9 - PubMed
    1. Diabetes. 1999 Sep;48(9):1706-12 - PubMed
    1. Int J Obes Relat Metab Disord. 2004 Dec;28(12):1535-40 - PubMed
    1. Cardiovasc Res. 2004 Jan 1;61(1):143-51 - PubMed
    1. Physiol Behav. 2003 Sep;79(4-5):795-801 - PubMed

Publication types

LinkOut - more resources