Aging sensitizes rapidly isolated hippocampal microglia to LPS ex vivo

J Neuroimmunol. 2010 Sep 14;226(1-2):181-4. doi: 10.1016/j.jneuroim.2010.05.022. Epub 2010 May 26.

Abstract

The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cytokines were assessed. 24 mo old animals exhibited a potentiated pro-inflammatory cytokine (IL-1β and IL-6) response to LPS and increased levels of CD11b, Iba-1 and MHCII irrespective of LPS treatment. The present results demonstrate that aging sensitizes hippocampal microglia to pro-inflammatory challenges.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aging / immunology*
  • Animals
  • CD11b Antigen / genetics
  • CD11b Antigen / metabolism
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism
  • Cytokines / genetics
  • Cytokines / metabolism
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects
  • Hippocampus / cytology*
  • Hippocampus / drug effects
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / metabolism
  • Lipopolysaccharides / pharmacology*
  • Male
  • Microfilament Proteins
  • Neuroglia / drug effects*
  • Neuroglia / immunology
  • Rats
  • Rats, Inbred F344

Substances

  • Aif1 protein, rat
  • CD11b Antigen
  • Calcium-Binding Proteins
  • Cytokines
  • Histocompatibility Antigens Class II
  • Lipopolysaccharides
  • Microfilament Proteins