Abstract
The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cytokines were assessed. 24 mo old animals exhibited a potentiated pro-inflammatory cytokine (IL-1β and IL-6) response to LPS and increased levels of CD11b, Iba-1 and MHCII irrespective of LPS treatment. The present results demonstrate that aging sensitizes hippocampal microglia to pro-inflammatory challenges.
Copyright © 2010 Elsevier B.V. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Aging / immunology*
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Animals
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CD11b Antigen / genetics
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CD11b Antigen / metabolism
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism
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Cytokines / genetics
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Cytokines / metabolism
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Dose-Response Relationship, Drug
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Gene Expression Regulation / drug effects
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Hippocampus / cytology*
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Hippocampus / drug effects
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / metabolism
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Lipopolysaccharides / pharmacology*
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Male
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Microfilament Proteins
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Neuroglia / drug effects*
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Neuroglia / immunology
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Rats
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Rats, Inbred F344
Substances
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Aif1 protein, rat
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CD11b Antigen
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Calcium-Binding Proteins
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Cytokines
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Histocompatibility Antigens Class II
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Lipopolysaccharides
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Microfilament Proteins