Metabolomic analysis of thermally injured and/or septic rats

Burns. 2010 Nov;36(7):992-8. doi: 10.1016/j.burns.2010.03.015. Epub 2010 May 26.


Introduction: Early diagnosis and treatment for thermal injury with septic complications continue to be a serious clinical problem. In this study, plasma biomarkers of rats in the burn and/or septic models were investigated with a metabolomic method.

Methods: Rat plasma samples were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Multivariate analysis, the principal components analysis (PCA), was used to validate metabolic changes. In addition, another multivariate method, the orthogonal partial least-squares analysis (OPLS), was used to profile potential biomarkers in models.

Results: Nine characteristic metabolites, including hypoxanthine, indoxyl sufate, glucuronic acid, gluconic acid, proline, uracil, nitrotyrosine, uric acid, and trihydroxy cholanoic acid were identified in models of thermal injury and/or sepsis.

Conclusion: These biomarkers were mainly involved in oxidative stress and tissue damage, and might supply evidence for distinguishing burned septic patients from non-septic ones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / blood
  • Animals
  • Biomarkers / blood
  • Burns / blood*
  • Carboxylic Acids / blood
  • Chromatography, High Pressure Liquid / methods
  • Disease Models, Animal
  • Heterocyclic Compounds / blood
  • Male
  • Mass Spectrometry
  • Metabolomics / methods
  • Rats
  • Rats, Sprague-Dawley
  • Sepsis / blood*
  • Sepsis / diagnosis


  • Amino Acids
  • Biomarkers
  • Carboxylic Acids
  • Heterocyclic Compounds