Adaptation of an enzymatic polyfructosan assay to clinical practice

Anal Biochem. 2010 Oct 15;405(2):266-8. doi: 10.1016/j.ab.2010.06.016. Epub 2010 Jun 10.


Inulin or polyfructosan clearance is regarded as the most accurate method of assessing the glomerular filtration rate. We propose an enzymatic method of polyfructosan determination based on the hydrolysis of polyfructosan into fructose by inulinase and the elimination of the interfering quantity of glucose by glucose oxidase. This spectrophotometric microplate formatted assay, which demonstrated very good specificity and reproducibility (within-run precision <1% and between-run precision <3.5%), is cheap and simple to perform and can be used by all analytical laboratories and in all clinical conditions.

MeSH terms

  • Clinical Enzyme Tests / methods*
  • Fructans / analysis*
  • Fructans / blood
  • Fructans / urine
  • Fructose / metabolism
  • Glomerular Filtration Rate*
  • Glycoside Hydrolases / metabolism
  • Hydrolysis
  • Inulin / metabolism
  • Reproducibility of Results


  • Fructans
  • Fructose
  • polyfructosan
  • Inulin
  • Glycoside Hydrolases
  • inulinase