Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death

FEBS Lett. 2010 Jul 16;584(14):3193-7. doi: 10.1016/j.febslet.2010.06.006. Epub 2010 Jun 10.


Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Death / drug effects
  • Cell Death / immunology
  • Coculture Techniques
  • Endothelial Cells / drug effects
  • Endothelial Cells / immunology
  • Endothelium / drug effects
  • Endothelium / immunology
  • Extracellular Space / drug effects
  • Extracellular Space / immunology*
  • Female
  • Hereditary Autoinflammatory Diseases
  • Humans
  • Interleukin-8 / immunology
  • Interleukin-8 / pharmacology
  • Neutrophils* / cytology
  • Neutrophils* / immunology
  • Neutrophils* / physiology
  • Pre-Eclampsia / immunology
  • Pregnancy


  • Interleukin-8