Gene expression profiling in nerve biopsy of vasculitic neuropathy

J Neuroimmunol. 2010 Aug 25;225(1-2):184-9. doi: 10.1016/j.jneuroim.2010.05.023. Epub 2010 Jun 12.

Abstract

To investigate molecular mechanisms of peripheral nerve vasculitis, gene expression patterns in archived frozen sural nerve biopsies from patients with vasculitic neuropathy were compared to control nerves by DNA microarray technology. There was a striking upregulation of mRNA of genes involved in immune system processes. Of special interest was the activation of immunoglobulin genes, such as IGLJ3, IGHG3, IGKC, and IGL, and of several chemokines, such as CXCL9 or CCR2. Genes involved in vascular proliferation or remodelling such as CXC31 and AIF were also upregulated. Among the downregulated genes were the Krüppel-Like Transcription Factors KLF2, KLF4 and the nuclear orphan receptor NR4A1 genes known to be involved in endothelial cell activation. Thus, this gene expression profile analysis revealed that in peripheral nerve vasculitis a prominent activation of immune response related genes as well as genes involved in vascular proliferation is taken place, while genes inhibiting endothelial cell activation are down regulated. These data point to interesting mechanistic clues to the molecular pathogenesis of vasculitic neuropathies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biopsy / methods
  • Child, Preschool
  • Female
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation / physiology*
  • Humans
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism
  • Male
  • Nuclear Receptor Subfamily 4, Group A, Member 1 / genetics
  • Nuclear Receptor Subfamily 4, Group A, Member 1 / metabolism
  • Peripheral Nervous System Diseases / complications
  • Peripheral Nervous System Diseases / genetics
  • Peripheral Nervous System Diseases / metabolism
  • Peripheral Nervous System Diseases / pathology*
  • Sural Nerve / immunology
  • Sural Nerve / metabolism*
  • Vasculitis / complications
  • Vasculitis / genetics
  • Vasculitis / metabolism
  • Vasculitis / pathology*

Substances

  • Kruppel-Like Transcription Factors
  • NR4A1 protein, human
  • Nuclear Receptor Subfamily 4, Group A, Member 1