Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes

Fertil Steril. 2010 Dec;94(7):2804-7. doi: 10.1016/j.fertnstert.2010.04.060. Epub 2010 Jun 9.


To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed through real-time reverse transcriptase-polymerase chain reaction eight genes encoding critically important proteins for embryo development and compared this partial transcriptome with that of freshly collected gametes isolated from the same women. The comparison of the molecular profiles demonstrated that our vitrification protocol does not alter the biomolecular quality of oocytes: in fact, between the two groups we found the absence of statistically significant variations. Accordingly, this cryopreservation technique might be helpful in preserving women's fertility.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Cell Separation
  • Cells, Cultured
  • Cryopreservation / methods
  • Embryonic Development / genetics
  • Female
  • Gene Expression Profiling
  • Germ Cells / cytology
  • Germ Cells / metabolism
  • Humans
  • Oocytes / cytology
  • Oocytes / metabolism*
  • RNA Stability / physiology
  • Validation Studies as Topic
  • Vitrification*