K-ras mutational status predicts poor prognosis in unresectable pancreatic cancer

Eur J Surg Oncol. 2010 Jul;36(7):657-62. doi: 10.1016/j.ejso.2010.05.014. Epub 2010 Jun 9.

Abstract

Objective: To determine the prognostic value of K-ras mutations in plasma DNA of unresectable pancreatic cancer patients.

Methods: Blood samples were collected from 91 patients with unresectable pancreatic cancer prior to treatment. K-ras gene was amplified from the circulating plasma DNA. Mutations were detected by direct sequencing. The relationship between the types of K-ras gene and prognosis of unresectable pancreatic cancer was evaluated.

Results: K-Ras codon 12 mutations were found in 30 of 91(33%) plasma DNA samples, 17mutations were c.35G>A (p.G12D), 11 were c.35G>T (p.G12V) and only 2 were c.34G>C (p.G12R)). K-ras codon 12 mutations could significantly reflect the clinical parameters, including TNM tumor staging (P=0.033) and liver metastasis (P=0.014). The median survival time of patients with K-ras mutations was shorter than that of patients with wild-type K-ras gene (3.9 months vs. 10.2 months, P<0.001). K-ras codon 12 mutation from plasma DNA was an independent negative prognostic factor for survival (hazard ratio, 7.39; 95% confidence interval, 3.69-14.89).

Conclusion: K-ras mutation in plasma DNA is a predictive biomarker for a poor prognosis of unresectable pancreatic cancer patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Asian Continental Ancestry Group / genetics*
  • China
  • Codon
  • DNA, Neoplasm / genetics
  • Female
  • Genes, ras*
  • Humans
  • Kaplan-Meier Estimate
  • Liver Neoplasms / secondary
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Mutation*
  • Neoplasm Staging
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / pathology
  • Pancreatic Neoplasms / therapy*
  • Predictive Value of Tests
  • Prognosis
  • Risk Assessment
  • Risk Factors
  • Treatment Outcome

Substances

  • Codon
  • DNA, Neoplasm