Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid

J Bacteriol. 2010 Aug;192(16):4251-5. doi: 10.1128/JB.00492-10. Epub 2010 Jun 11.


L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.

MeSH terms

  • Aminobutyrates / metabolism*
  • Anti-Bacterial Agents / biosynthesis*
  • Antimetabolites / metabolism*
  • Biosynthetic Pathways / genetics*
  • Gene Order
  • Genes, Bacterial
  • Multigene Family*
  • Mutagenesis, Site-Directed
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / metabolism*


  • Aminobutyrates
  • Anti-Bacterial Agents
  • Antimetabolites
  • 2-amino-4-methoxy-3-butenoic acid