Comparison of cellular response to anorganic bone matrix/cell binding peptide and allogenic cranial bone after sinus augmentation in rhesus monkeys

J Oral Implantol. 2011 Apr;37(2):233-45. doi: 10.1563/AAID-JOI-D-09-00081. Epub 2010 Jun 14.


This study compared cellular responses of maxillary sinuses after augmentation with anorganic bovine-derived hydroxyapatite matrix linked to the cell binding polypeptide P-15 (ABM/P-15) or PepGen P-15 and allogenic freeze-dried cranial bone slabs. Five adult Macaque fascicularis monkeys were used. On one side, the floor of the sinus was augmented with ABM/P-15, while the other side was augmented with 2 cranial bone slabs. Trephine bone biopsies were obtained 6, 12, and 24 weeks postgrafting. Animals were sacrificed 8 months after grafting. Soft X-ray microradiography was used to determine bone density. The volume fraction (Vv) of regenerated bone, the number of mesenchymal cells, and the numbers of proliferating cell nuclear antigen (PCNA)- and alkaline phosphatase-positive cells at different augmentation sites were measured and compared. Basal bone heights were calculated at surgery and compared with total heights of the augmented sinus floors 8 months postgrafting. Bone formation, number of mesenchymal cells, PCNA index, and alkaline phosphatase index were significantly higher for the ABM/P-15 side than for the allogenic bone-augmented side. Both sides ended with a significant increase in bone height. The PCNA index decreased significantly over time (P < .05), while the alkaline phosphatase index increased significantly (P < .05) over time on both sides. Both graft materials have led to significant augmentation of the floor of the maxillary sinus with new bone; however, new bone formation and maturation were faster on the ABM/P-15 sites.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone Density / drug effects
  • Bone Matrix / transplantation*
  • Bone Regeneration / drug effects*
  • Bone Substitutes* / pharmacology
  • Bone Transplantation / methods*
  • Cattle
  • Cell Adhesion / drug effects
  • Cell Differentiation
  • Durapatite
  • Macaca mulatta
  • Maxillary Sinus / surgery*
  • Mesoderm / cytology
  • Microradiography
  • Oral Surgical Procedures, Preprosthetic / methods*
  • Proliferating Cell Nuclear Antigen / biosynthesis


  • Bone Substitutes
  • PepGen P-15
  • Proliferating Cell Nuclear Antigen
  • Durapatite
  • Alkaline Phosphatase