Effect of fluoride and of calcium sodium phosphosilicate toothpastes on pre-softened dentin demineralization and remineralization in vitro

J Dent. 2010 Aug;38(8):671-7. doi: 10.1016/j.jdent.2010.05.010. Epub 2010 Jun 9.


Objectives: The purpose of this in vitro study was to evaluate the effect of toothpastes containing sodium fluoride in different concentrations or a calcium sodium phosphosilicate system on pre-softened dentin demineralization and remineralization.

Methods: During a ten day pH-cycling protocol, pre-softened bovine root dentin slabs were immersed twice daily, after the demineralization periods, for 2min, in the following toothpaste slurries: (a) non-fluoridated (control), (b) 7.5% calcium sodium phosphosilicate, (c) 1450 ppm F, (d) 2800 ppm F and (e) 5000 ppm F. Subsequently, the specimens were subjected to a 15-h acid resistance test. Surface microhardness was assessed initially and during the pH-cycling and the acid resistance test period. Repeated measurements in each group were analyzed through appropriate regression models for longitudinal data.

Results: All fluoride groups, during pH cycling, showed significantly less microhardness loss (p < or = 0.010, p < or = 0.002, p < or = 0.002) and subsequently exhibited increased acid resistance (p < or = 0.010, p < or = 0.001, p < or = 0.001) compared to the control. The 5000 ppm and 2800 ppm F toothpastes, inhibited demineralization significantly more effectively than the 1450 ppm F (p < or = 0.001, p < or = 0.030) and the calcium sodium phosphosilicate toothpaste (p < or = 0.001), while no significant differences were found between the two high fluoride groups (p=0.130). The calcium sodium phosphosilicate toothpaste, during pH cycling showed a difference that approached statistical significance compared to control (p=0.079), but its acid resistance behavior was similar to control (p=0.610).

Conclusions: Under these experimental conditions, the high fluoride toothpastes promoted remineralization and inhibited demineralization more effectively, than the 1450 ppm F, the non-fluoridated (control) and the calcium sodium phosphosilicate toothpastes.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Cariostatic Agents / pharmacology
  • Cariostatic Agents / therapeutic use*
  • Cattle
  • Dentin / drug effects*
  • Dentin / pathology
  • Drug Combinations
  • Glass*
  • Hardness / drug effects
  • Hydrogen-Ion Concentration
  • Linear Models
  • Silicates / pharmacology
  • Silicates / therapeutic use*
  • Sodium Fluoride / pharmacology
  • Sodium Fluoride / therapeutic use*
  • Tooth Demineralization / prevention & control*
  • Tooth Remineralization / methods*
  • Tooth Root / drug effects
  • Tooth Root / pathology
  • Toothpastes / chemistry
  • Toothpastes / pharmacology
  • Toothpastes / therapeutic use*


  • Cariostatic Agents
  • Drug Combinations
  • NovaMin
  • Silicates
  • Toothpastes
  • Sodium Fluoride