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, 107 (26), 11657-8

Breaking News: Plants Mutate Right on Target

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Breaking News: Plants Mutate Right on Target

Holger Puchta et al. Proc Natl Acad Sci U S A.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Target for ZFN, with the two different subunits of the enzyme drawn below and above the target sequence. The sequence in between is being cut, upon dimerization of the enzyme, yielding a 4-bp-long 5′ overhang. This can be repaired by the host-specific NHEJ activity, usually leaving small deletions or insertions behind. Alternatively, a homologous rescue construct, supplied at the time of enzyme activation, is used by the host's homologous recombination activity, to replace the endogenous sequence.

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