Rapid typing of DNA sequence polymorphism at the HLA-DRB1 locus using the polymerase chain reaction and nonradioactive oligonucleotide probes

Hum Immunol. 1991 Mar;30(3):190-201. doi: 10.1016/0198-8859(91)90034-7.


An HLA-DR typing system that uses sequence-specific oligonucleotide (SSO) probes conjugated to horseradish peroxidase (HRP) for analyzing DRB alleles amplified by the polymerase chain reaction has been developed. Using 25 HRP-SSO probes and two primer pairs for generic and for DRB1 locus-specific amplification, we can distinguish 31 of 34 HLA-DRB1 alleles. This procedure is suitable for typing heterozygous samples from a variety of sources, including cDNA templates, and can detect in a simple and rapid dot-blot format allelic variants not distinguishable by serological methods. It should prove valuable for tissue typing, determining individual identity, and studies of disease susceptibility.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / genetics*
  • DNA Probes
  • Exons
  • HLA-DR Antigens / classification
  • HLA-DR Antigens / genetics*
  • HLA-DRB1 Chains
  • Histocompatibility Antigens Class II / classification
  • Histocompatibility Antigens Class II / genetics*
  • Histocompatibility Testing
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic


  • DNA Probes
  • HLA-DR Antigens
  • HLA-DRB1 Chains
  • Histocompatibility Antigens Class II
  • DNA