Manipulation of FASTQ data with Galaxy

Bioinformatics. 2010 Jul 15;26(14):1783-5. doi: 10.1093/bioinformatics/btq281. Epub 2010 Jun 18.


Summary: Here, we describe a tool suite that functions on all of the commonly known FASTQ format variants and provides a pipeline for manipulating next generation sequencing data taken from a sequencing machine all the way through the quality filtering steps.

Availability and implementation: This open-source toolset was implemented in Python and has been integrated into the online data analysis platform Galaxy (public web access:; download: Two short movies that highlight the functionality of tools described in this manuscript as well as results from testing components of this tool suite against a set of previously published files are available at

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Databases, Factual
  • Genomics / methods*
  • Internet
  • Sequence Analysis, DNA / methods
  • Software*
  • User-Computer Interface