Spatial organization of the flow of genetic information in bacteria

Nature. 2010 Jul 1;466(7302):77-81. doi: 10.1038/nature09152. Epub 2010 Jun 20.


Eukaryotic cells spatially organize mRNA processes such as translation and mRNA decay. Much less is clear in bacterial cells where the spatial distribution of mature mRNA remains ambiguous. Using a sensitive method based on quantitative fluorescence in situ hybridization, we show here that in Caulobacter crescentus and Escherichia coli, chromosomally expressed mRNAs largely display limited dispersion from their site of transcription during their lifetime. We estimate apparent diffusion coefficients at least two orders of magnitude lower than expected for freely diffusing mRNA, and provide evidence in C. crescentus that this mRNA localization restricts ribosomal mobility. Furthermore, C. crescentus RNase E appears associated with the DNA independently of its mRNA substrates. Collectively, our findings show that bacteria can spatially organize translation and, potentially, mRNA decay by using the chromosome layout as a template. This chromosome-centric organization has important implications for cellular physiology and for our understanding of gene expression in bacteria.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Caulobacter crescentus / cytology
  • Caulobacter crescentus / genetics*
  • Caulobacter crescentus / metabolism*
  • Chaperonins / genetics
  • Chromosomes, Bacterial / genetics
  • Chromosomes, Bacterial / metabolism
  • DNA, Bacterial / metabolism
  • Diffusion
  • Endoribonucleases / metabolism
  • Escherichia coli / cytology
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Gene Expression Regulation, Bacterial
  • In Situ Hybridization, Fluorescence
  • Lac Operon / genetics
  • Protein Biosynthesis
  • RNA Stability
  • RNA Transport*
  • RNA, Bacterial / analysis
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Ribosomes / metabolism
  • Transcription, Genetic / genetics


  • Bacterial Proteins
  • DNA, Bacterial
  • GroESL protein, Bacteria
  • RNA, Bacterial
  • RNA, Messenger
  • Endoribonucleases
  • ribonuclease E
  • Chaperonins