Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation

Nat Cell Biol. 2010 Jul;12(7):719-27. doi: 10.1038/ncb2075. Epub 2010 Jun 20.

Abstract

Heterochromatin protein 1 (HP1) has an essential role in heterochromatin formation and mitotic progression through its interaction with various proteins. We have identified a unique HP1alpha-binding protein, POGZ (pogo transposable element-derived protein with zinc finger domain), using an advanced proteomics approach. Proteins generally interact with HP1 through a PxVxL (where x is any amino-acid residue) motif; however, POGZ was found to bind to HP1alpha through a zinc-finger-like motif. Binding by POGZ, mediated through its zinc-finger-like motif, competed with PxVxL proteins and destabilized the HP1alpha-chromatin interaction. Depletion experiments confirmed that the POGZ HP1-binding domain is essential for normal mitotic progression and dissociation of HP1alpha from mitotic chromosome arms. Furthermore, POGZ is required for the correct activation and dissociation of Aurora B kinase from chromosome arms during M phase. These results reveal POGZ as an essential protein that links HP1alpha dissociation with Aurora B kinase activation during mitosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aurora Kinase B
  • Aurora Kinases
  • Blotting, Western
  • Cell Line
  • Chromatids / genetics
  • Chromatids / metabolism
  • Chromatin / metabolism
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Fluorescence Recovery After Photobleaching
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Kinetochores / metabolism
  • Mass Spectrometry
  • Microscopy, Fluorescence
  • Mitosis / genetics
  • Mitosis / physiology*
  • Protein Binding
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA Interference
  • Transposases / genetics
  • Transposases / metabolism*
  • Two-Hybrid System Techniques

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • heterochromatin-specific nonhistone chromosomal protein HP-1
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases
  • PogZ protein, human
  • Transposases