A polysaccharide extracted from Grifola frondosa enhances the anti-tumor activity of bone marrow-derived dendritic cell-based immunotherapy against murine colon cancer

Cancer Immunol Immunother. 2010 Oct;59(10):1531-41. doi: 10.1007/s00262-010-0880-7. Epub 2010 Jun 20.


We previously isolated the novel heteropolysaccharide maitake Z-fraction (MZF) from the maitake mushroom (Grifola frondosa), and demonstrated that MZF significantly inhibited tumor growth by inducing cell-mediated immunity. In this study, we demonstrated that MZF upregulated the expression of CD80, CD86, CD83, and MHC II on bone marrow-derived dendritic cells (DCs) and significantly increased interleukin-12 (IL-12) and tumor necrosis factor-alpha production by DCs in a dose-dependent manner. MZF-treated DCs significantly stimulated both allogeneic and antigen-specific syngenic T cell responses and enhanced antigen-specific interferon-gamma (IFN-gamma) production by syngenic CD4(+) T cells; however, MZF-treated DCs did not affect IL-4 production. Furthermore, the enhancement of IFN-gamma production in CD4(+) T cells, which was induced by MZF-treated DCs, was completely inhibited by the addition of an anti-IL-12 antibody. These results indicate that MZF induced DC maturation and antigen-specific Th1 response by enhancing DC-produced IL-12. We also demonstrated that DCs pulsed with colon-26 tumor lysate in the presence of MZF induced both therapeutic and preventive effects on colon-26 tumor development in BALB/c mice. These results suggest that MZF could be a potential effective adjuvant to enhance immunotherapy using DC-based vaccination.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology*
  • Bone Marrow / immunology*
  • Colonic Neoplasms / therapy*
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology
  • Female
  • Flow Cytometry
  • Grifola / chemistry*
  • Immunotherapy*
  • Mice
  • Mice, Inbred BALB C
  • Polysaccharides / chemistry*
  • Polysaccharides / pharmacology


  • Antineoplastic Agents
  • Polysaccharides