Paullinia cupana Mart. var. sorbilis, guarana, increases survival of Ehrlich ascites carcinoma (EAC) bearing mice by decreasing cyclin-D1 expression and inducing a G0/G1 cell cycle arrest in EAC cells

Phytother Res. 2011 Jan;25(1):11-6. doi: 10.1002/ptr.3216.

Abstract

The objective of this work is to report the antiproliferative effect of P. cupana treatment in Ehrlich Ascites Carcinoma (EAC)-bearing animals. Female mice were treated with three doses of powdered P. cupana (100, 1000 and 2000 mg/kg) for 7 days, injected with 10(5) EAC cells and treated up to day 21. In addition, a survival experiment was carried out with the same protocol. P. cupana decreased the ascites volume (p = 0.0120), cell number (p = 0.0004) and hemorrhage (p = 0.0054). This occurred through a G1-phase arrest (p < 0.01) induced by a decreased gene expression of Cyclin D1 in EAC cells. Furthermore, P. cupana significantly increased the survival of EAC-bearing animals (p = 0.0012). In conclusion, the P. cupana growth control effect in this model was correlated with a decreased expression of cyclin D1 and a G1 phase arrest. These results reinforce the cancer therapeutic potential of this Brazilian plant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Ehrlich Tumor / drug therapy*
  • Carcinoma, Ehrlich Tumor / mortality
  • Carcinoma, Ehrlich Tumor / pathology
  • Cell Cycle / drug effects*
  • Cell Proliferation / drug effects*
  • Cyclin D1 / metabolism*
  • Cytostatic Agents / pharmacology
  • Cytostatic Agents / therapeutic use*
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Paullinia*
  • Phytotherapy*
  • Plant Preparations / pharmacology
  • Plant Preparations / therapeutic use*
  • Survival Analysis

Substances

  • Cytostatic Agents
  • Plant Preparations
  • Cyclin D1