Abstract
A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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AKR murine leukemia virus / analysis
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AKR murine leukemia virus / growth & development
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AKR murine leukemia virus / isolation & purification*
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Centrifugation, Density Gradient
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Chromatography, Agarose / methods*
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Chromatography, Gel / methods*
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Gammaretrovirus / analysis
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Gammaretrovirus / growth & development
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Gammaretrovirus / isolation & purification*
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Glycoproteins / analysis
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Leukemia Virus, Murine / isolation & purification*
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Peptides / analysis
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Viral Plaque Assay
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Viral Proteins / analysis
Substances
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Glycoproteins
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Peptides
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Viral Proteins