Since 2001, the United States government has spent substantial resources on preparing the nation against a bioterrorist attack. Earlier articles in this series have analyzed civilian biodefense funding by the federal government for fiscal years (FY) 2001 through FY2010. This article updates those figures with budgeted amounts for FY2011, specifically analyzing the budgets and allocations for biodefense at the Departments of Health and Human Services, Defense, Homeland Security, Agriculture, Commerce, and State; the Environmental Protection Agency; and the National Science Foundation. This article also includes an updated assessment of the proportion of biodefense funding provided for programs that address multiple public health, healthcare, national security, and international security issues in addition to biodefense. The FY2011 federal budget for civilian biodefense totals $6.48 billion. Of that total, $5.90 billion (91%) is budgeted for programs that have both biodefense and nonbiodefense goals and applications, and $577.9 million (9%) is budgeted for programs that deal strictly with biodefense.