Flax and Pumpkin seeds mixture ameliorates diabetic nephropathy in rats

Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2407-12. doi: 10.1016/j.fct.2010.05.079. Epub 2010 Jun 4.


This study investigated the hypoglycemic and antioxidant effects of Flax and Pumpkin seeds mixture on the kidney of alloxan-induced diabetic rats. Animals were allocated into three groups of six rats each: a control group (CD), a diabetic group (DD) and diabetic rats fed with Flax and Pumpkin seeds mixture (DMS) group. The DD rats showed a significant increase of glycemia and lipid parameters such as total lipid, total cholesterol and triglycerides levels compared to those of the control group (CD). In addition, plasma and kidney malonaldialdehyde levels (MDA) were significantly increased compared to (CD) group. Antioxidant enzyme activities such as catalase (CAT), superoxide dismutase (SOD) and non-enzymatic levels of reduced glutathione (GSH) significantly decreased in the plasma and kidney of diabetic rats compared to those of controls. Diet supplemented with Flax and Pumpkin seeds mixture ameliorated the antioxidant enzymes activities observed in diabetic rats and significantly decreased MDA levels. Kidney histological sections, showed glomerular hypertrophy and tubular dilatation. In DMS rats, these histopathological changes were less prominent. Our results suggest that Flax and Pumpkin seeds mixture supplemented in diet of diabetic rats may be helpful to prevent diabetes and its complications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • Body Weight / drug effects
  • Catalase / metabolism
  • Creatinine / metabolism
  • Cucurbita / chemistry*
  • Diabetes Mellitus, Experimental / complications
  • Diabetic Nephropathies / drug therapy*
  • Flax / chemistry*
  • Glucose Tolerance Test
  • Glutathione / metabolism
  • Hypoglycemic Agents / therapeutic use
  • Insulin / blood
  • Lipids / blood
  • Male
  • Malondialdehyde / analysis
  • Malondialdehyde / metabolism
  • Phytotherapy*
  • Rats
  • Rats, Wistar
  • Seeds / chemistry
  • Superoxide Dismutase / metabolism
  • Urea / metabolism
  • Uric Acid / metabolism


  • Blood Glucose
  • Hypoglycemic Agents
  • Insulin
  • Lipids
  • Uric Acid
  • Malondialdehyde
  • Urea
  • Creatinine
  • Catalase
  • Superoxide Dismutase
  • Glutathione