The load that maximizes power output in the jump squat (JS) in college-aged athletic males has been reported to be 0% of 1 repetition maximum [1RM] squat strength) or in other words body mass. No data exist concerning adolescent athletic males. In addition, strength levels have been theorized to possibly affect the load that maximizes power output in the JS. The purpose of this investigation was to identify the load that maximizes power output in the JS in adolescent athletic men, and concurrently describe their strength level and its effect on the load that maximizes power output. Eleven high-school male athletes were tested on 2 occasions, first determining their 1RM in the squat (1RM = 141.14 ± 28.08 kg; squat 1RM-to-body mass ratio = 1.76 ± 0.15) and then performing JS testing at loads equal to 0% (body mass), 20, 40, 60, and 80% of squat 1RM. Peak power (PP), peak force, peak velocity (PV), and peak displacement were measured at each load. Jump squat at the 0% load produced significantly (p ≤ 0.05) higher PP, PV, and peak displacement in comparison with the 40, 60, and 80% loading conditions. It was concluded that the load that maximizes power output in the JS is 0% of 1RM in adolescent athletic men, the same as found in college-aged athletic men. In addition, strength level relative to body mass did not affect the load that maximized power output. Practically, when devising a training program to increase PP, it is important to include JSs at body mass along with traditional strength training at heavier loads to increase power output across the entire loading spectrum.