A method to site-specifically introduce methyllysine into proteins in E. coli

Chem Commun (Camb). 2010 Aug 14;46(30):5506-8. doi: 10.1039/c0cc00108b. Epub 2010 Jun 22.


A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acyl-tRNA Synthetases / genetics*
  • Escherichia coli / genetics*
  • Escherichia coli Proteins / genetics*
  • Lysine / analogs & derivatives*
  • Lysine / genetics
  • Mutation
  • RNA, Transfer / genetics*


  • Escherichia coli Proteins
  • 2-methyllysine
  • RNA, Transfer
  • Amino Acyl-tRNA Synthetases
  • Lysine