Abstract
MUC1 glycopeptide was efficiently coupled to glycosylphosphatidylinositol (GPI) derivatives by sortase A (SrtA), verifying that SrtA can accept sterically hindered glycopeptide as substrate for ligation with GPIs. This work has established a practical method for the chemoenzymatic synthesis of GPI-linked glycopeptides.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Aminoacyltransferases / metabolism
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Bacterial Proteins / metabolism
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Biocatalysis
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Carbohydrate Sequence
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Cysteine Endopeptidases / metabolism
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Glycopeptides / biosynthesis*
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Glycopeptides / chemistry
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Glycosylphosphatidylinositols / chemistry*
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Molecular Sequence Data
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Mucin-1 / chemistry
Substances
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Bacterial Proteins
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Glycopeptides
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Glycosylphosphatidylinositols
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Mucin-1
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Aminoacyltransferases
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sortase A
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Cysteine Endopeptidases