Objective: To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve.
Methods: The molecular biological technique-random amplified polymorphic DNA (RAPD) were used.
Results: 104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia. 422 DNA bands were amplified by 12 primers, 55.69% products were found to be polymorphic. Base on UPGMA cluster analysis, a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant, but not to geographic regions.
Conclusion: There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.