Human adult germline stem cells in question

Nature. 2010 Jun 24;465(7301):E1; discussion E3. doi: 10.1038/nature09089.


Conrad et al. have generated human adult germline stem cells (haGSCs) from human testicular tissue, which they claim have similar pluripotent properties to human embryonic stem cells (hESCs). Here we investigate the pluripotency of haGSCs by using global gene-expression analysis based on their gene array data and comparing the expression of pluripotency marker genes in haGSCs and hESCs, and in haGSCs and human fibroblast samples derived from different laboratories, including our own. We find that haGSCs and fibroblasts have a similar gene-expression profile, but that haGSCs and hESCs do not. The pluripotency of Conrad and colleagues' haGSCs is therefore called into question.

Publication types

  • Comment
  • Comparative Study

MeSH terms

  • Adult
  • Animals
  • Biomarkers / analysis
  • Biopsy
  • Cells, Cultured
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Gene Expression Profiling
  • Germ Cells / cytology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism*
  • Male
  • Mice
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Reproducibility of Results
  • Testis / cytology


  • Biomarkers
  • RNA, Messenger

Associated data

  • GEO/GSE10831
  • GEO/GSE11350
  • GEO/GSE12583
  • GEO/GSE15148
  • GEO/GSE15322