Small peptide (P-15) bone substitute efficacy in a rabbit cancellous bone model

J Biomed Mater Res B Appl Biomater. 2010 Aug;94(2):463-8. doi: 10.1002/jbm.b.31676.


P-15 is a synthetic 15-amino acid residue identical to the cell binding domain of type I collagen. P-15 can be adsorbed onto anorganic bovine bone mineral (ABM) and will enhance cell attachment and subsequent cell activation. Although ABM/P-15 has been studied as a bone graft substitute in the oral cavity, its use in orthopedic models has been limited. Thus, this study investigated the efficacy of ABM/P-15 treatment in a rabbit model of long bone cancellous healing. Defects were created in the distal femurs and proximal medial tibiae of rabbits and were filled with either ABMP/P-15 suspended in hydrogel, ABM alone suspended in hydrogel, hydrogel carrier alone, or no graft material. Rabbits were sacrificed at 1, 2, 4, or 8 weeks postsurgery, and the femurs and tibiae were harvested. Histomorphometric analyses indicated that defects treated with ABM/P-15 had significantly larger areas of new bone formation than the other three treatments at 2 and 8 weeks postsurgery. ABM/P-15 treated defects also had significantly more bone growth than defects left empty or filled with ABM alone at 4 weeks postsurgery. Furthermore, histological examination did not reveal acute inflammatory infiltrate cells in any of the treatment conditions. These results are consistent with the findings of ABM/P-15 use in human oral-maxillofacial studies and in large animal spine fusion models.

MeSH terms

  • Animals
  • Bone Substitutes / chemistry*
  • Bone Substitutes / therapeutic use
  • Collagen / therapeutic use*
  • Femur / growth & development
  • Femur / injuries
  • Femur / surgery
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Implants, Experimental / standards*
  • Peptide Fragments / therapeutic use*
  • Rabbits
  • Tibia / growth & development
  • Tibia / injuries
  • Tibia / surgery
  • Treatment Outcome


  • Bone Substitutes
  • Peptide Fragments
  • cell-binding peptide P-15
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Collagen