Backbone assignment of the catalytic core of a Y-family DNA polymerase

Biomol NMR Assign. 2010 Oct;4(2):207-9. doi: 10.1007/s12104-010-9244-7. Epub 2010 Jun 29.


Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses DNA lesions. Here, we report the assignments for the backbone nitrogen, carbon, and amide proton NMR signals of Dpo4's catalytic core consisting of the finger, palm, and thumb domains. Our work provides the basis for further NMR spectroscopic studies of the interactions among Dpo4, DNA, and an incoming nucleotide.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Catalytic Domain*
  • DNA Polymerase beta / chemistry*
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Structure, Secondary
  • Sulfolobus solfataricus / enzymology*


  • DNA Polymerase beta