Large scale preparation of human MHC class II+ integrin beta(1)+ Tregs

J Immunol Methods. 2010 Aug 31;360(1-2):96-102. doi: 10.1016/j.jim.2010.06.014. Epub 2010 Jun 25.


The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4 Antigens / biosynthesis
  • Cells, Cultured
  • Flow Cytometry
  • Forkhead Transcription Factors / biosynthesis
  • High-Throughput Screening Assays
  • Histocompatibility Antigens Class II / biosynthesis*
  • Humans
  • Immune Tolerance
  • Immunomagnetic Separation*
  • Integrin alpha4beta1 / biosynthesis*
  • Interleukin-2 Receptor alpha Subunit / biosynthesis
  • Leukapheresis
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism*
  • T-Lymphocyte Subsets / pathology
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / metabolism*
  • T-Lymphocytes, Regulatory / pathology


  • CD4 Antigens
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Histocompatibility Antigens Class II
  • Integrin alpha4beta1
  • Interleukin-2 Receptor alpha Subunit