Determination of L-serine-O-phosphate in rat and mouse brain tissue using high-performance liquid chromatography and fluorimetric detection

Anal Biochem. 2010 Oct 15;405(2):260-2. doi: 10.1016/j.ab.2010.06.024. Epub 2010 Jun 17.


L-Serine-O-phosphate (L-SOP), the precursor of l-serine, is an agonist at group III metabotropic glutamate receptors. Despite the interest in L-SOP, very few articles have reported its brain levels. Here we report a convenient and reproducible method for simultaneous analysis of L-SOP and several other important amino acids in brain tissue using high-performance liquid chromatography (HPLC) with fluorimetric detection after derivatization with o-phthaldialdehyde and N-isobutyl-L-cysteine. Analyses were carried out in rat whole brain and cerebellum and in mouse whole brain, forebrain, amygdala, and prefrontal cortex. The method should be useful for future comprehensive neurochemical and pharmacological studies on neuropsychiatric disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Brain Chemistry*
  • Cerebellum
  • Chromatography, High Pressure Liquid / methods*
  • Fluorometry / methods*
  • Mice
  • Mice, Inbred C57BL
  • Phosphoserine / analysis*
  • Prefrontal Cortex / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Serine / metabolism


  • Phosphoserine
  • Serine