Expression of functional GPR35 in human iNKT cells

Biochem Biophys Res Commun. 2010 Jul 30;398(3):420-5. doi: 10.1016/j.bbrc.2010.06.091. Epub 2010 Jun 25.

Abstract

The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from alpha-galactosylceramide (alpha-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins. In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / metabolism*
  • GTP-Binding Protein alpha Subunits, Gi-Go / metabolism
  • Humans
  • Interleukin-4 / metabolism
  • Kynurenic Acid / pharmacology
  • Natural Killer T-Cells / metabolism*
  • Pertussis Toxin / pharmacology
  • Purinones / pharmacology
  • Receptors, G-Protein-Coupled / agonists
  • Receptors, G-Protein-Coupled / biosynthesis*
  • Receptors, G-Protein-Coupled / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • GPR35 protein, human
  • Purinones
  • Receptors, G-Protein-Coupled
  • Interleukin-4
  • Pertussis Toxin
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • zaprinast
  • Kynurenic Acid