Novel Candidate Substrates of AMP-activated Protein Kinase Identified in Red Blood Cell Lysates

Biochem Biophys Res Commun. 2010 Jul 23;398(2):296-301. doi: 10.1016/j.bbrc.2010.06.084. Epub 2010 Jun 25.

Abstract

AMPK is a metabolic stress-sensing kinase with important functions for red blood cell (RBC) survival. By using a proteomic approach, we identified putative AMPK targets in hemoglobin-depleted lysates of RBC, including metabolic enzymes, cytoskeletal proteins and enzymes involved in the oxidative stress response. These data tie in with the phenotypic observations of AMPKalpha1-deficient RBC and provide reference for future studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP-Activated Protein Kinases / chemistry
  • AMP-Activated Protein Kinases / metabolism*
  • Animals
  • Chromatography, Affinity
  • Enzyme Activation
  • Erythrocytes / enzymology*
  • Hemoglobins / chemistry
  • Hemoglobins / isolation & purification
  • Humans
  • Mice
  • Nickel / chemistry
  • Substrate Specificity

Substances

  • Hemoglobins
  • Nickel
  • AMP-Activated Protein Kinases